调节性T细胞(Treg)检测试剂盒

1129 人阅读发布时间：2014-06-04 10:16

天然CD4+ CD25+调节性T细胞( regulatory Tcell，Treg )为一类具有免疫抑制作用的T细胞，约占外周CD4+ T细胞的5～10%。该类细胞以"主动"的方式参与免疫应答／免疫耐受的调控，不仅参与自身免疫耐受调节，而且在肿瘤免疫和移植免疫中也具有重要的作用。Foxp3( forkhead box P3，Scurfin)是目前公认的Treg细胞最特征性的标志，其表达及功能与Treg细胞密切相关。Foxp3主要表达于胸腺、脾脏和淋巴结等淋巴器官和组织，主要通过流式细胞术来检测，美国eBioscience公司是最早拥有Foxp3流式检测抗体的公司之一，公司通过不断改进和优化，建立了一套完美的针对Foxp3流式检测特殊的试剂和方案。

供货商	产品编号	产品名称	规格	成分
eBioscience	88-4994-40	Human/Non-Human Primate Regulatory T Cell Staining Kit #2 Kit	Kit (25 tests)	FITC anti-human CD4 (OKT4): 25 tests. PE anti-human CD25 (BC96): 25 tests. APC anti-human Foxp3 (PCH101): 25 tests. APC Rat IgG2a isotype control: 25 tests. Normal Rat Serum: 100 µl. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供：Isotype controls for anti-- CD4(mouse IgG1, cat. 11‐4714) and anti‐ CD25 (mouse IgG1, cat. 12‐4714)
eBioscience	88-4999-40	Human/Non-Human Primate Regulatory T Cell Staining Kit #1Kit	Kit (25 tests)	FITC anti-human CD4 (OKT4): 25 tests. APC anti-human CD25 (BC96): 25 tests. PE anti-human Foxp3 (PCH101): 25 tests. PE Rat IgG2a isotype control: 25 tests. Normal Rat Serum: 100 µl. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供：Isotype controls for anti‐ CD4 (mouse IgG1, cat. 11‐4714) and anti‐ CD25 (mouse IgG1, cat. 17‐4714)
eBioscience	88-8111-40	Mouse Regulatory T Cell Staining Kit #1 Kit	Kit (50 tests)	FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test. APC anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test. PE anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test. PE Rat IgG2a isotype control: 50 µg. Use at 0.5 µg/test. Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg. Use at 1-5 µg/test. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供：Isotype controls for anti- CD4 (rat IgG2a, cat. 11-4321) and anti- CD25 (rat IgG1, cat. 17-4301)
eBioscience	88-8115-40	Mouse Regulatory T Cell Staining Kit #3 Kit	Kit	FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test. PE anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test. PE-Cy5 anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test. PE-Cy5 Rat IgG2a isotype control: 25 µg. Use at 0.5 µg/test. Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg. Use at 1-5 µg/test eBioscience Flow Cytometry Staining Buffer: 200 ml eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供：Isotype controls for anti- CD4 (rat IgG2a, cat. 11-4321) and anti- CD25 (rat IgG1, cat. 12-4301)
eBioscience	88-8118-40	Mouse Regulatory T Cell Staining Kit #2 Kit	Kit	FITC anti-mouse CD4 (RM4-5): 50 µg. Use at 0.125 µg/test. PE anti-mouse CD25 (PC61.5): 50 µg. Use at 0.06 µg/test. APC anti-mouse/rat Foxp3 (FJK-16s): 25 µg. Use at 0.5 µg/test. APC Rat IgG2a isotype control: 50 µg. Use at 0.5 µg/test. Affinity Purified anti-mouse CD16/32 (Fc Block): 50 µg Use at 2-5 µg/test. Flow Cytometry Staining Buffer: 200 ml Fixation/Permeabilization Concentrate: 30 ml.Fixation/Permeabilization Diluent: 100 ml. Store at 2-8°C. Permeabilization Buffer (10X): 100 ml. 未提供：Isotype controls for anti- CD4 (rat IgG2a, Cat. No. 11-4321) and anti- CD25 (rat IgG1, Cat. No. 12-4301)
eBioscience	88-8995-40	Human Regulatory T Cell Staining Kit #3 Kit	Kit	Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests. PE-Cy5 anti-human Foxp3 (PCH101): 25 tests. PE-Cy5 Rat IgG2a isotype control: 25 µg. Normal Rat Serum: 100 µl. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供： 12-4714 Mouse IgG1 K Isotype Control PE 11-4714 Mouse IgG1 K Isotype Control FITC
eBioscience	88-8998-40	Human Regulatory T Cell Staining Kit #2Kit	Kit	Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests. APC anti-human Foxp3 (PCH101): 25 tests. APC Rat IgG2a isotype control: 25 tests. Normal Rat Serum: 100 µl. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供： 12-4714 Mouse IgG1 K Isotype Control PE 11-4714 Mouse IgG1 K Isotype Control FITC
eBioscience	88-8999-40	Human Regulatory T Cell Staining Kit Kit	Kit	Cocktail of FITC anti-human CD4 (RPA-T4) and APC anti-human CD25 (BC96): 25 tests. PE anti-human Foxp3 (PCH101): 25 tests. PE Rat IgG2a isotype control: 25 tests. Normal Rat Serum: 100 µl. eBioscience Flow Cytometry Staining Buffer: 200 ml. eBioscience Fixation/Permeabilization Concentrate: 30 ml. eBioscience Fixation/Permeabilization Diluent: 100 ml. eBioscience Permeabilization Buffer (10X): 100 ml. 未提供： 11-4714 Mouse IgG1 K Isotype Control FITC 17-4714 Mouse IgG1 K Isotype Control APC
eBioscience	88-8996-40	Human Regulatory T Cell Whole Blood Staining Kit	Kit	eBioscience 1X RBC Lysis Buffer: 200 ml eBioscience Flow Cytometry Staining Buffer: 600 ml eBioscience Fixation/Permeabilization Concentrate: 30 ml eBioscience Fixation/Permeabilization Diluent: 100 ml PE anti-human Foxp3 (PCH101): 25 tests 未提供： CD4 抗体和CD25抗体及其同型对照 12-4321 Rat IgG2a K Isotype Control PE (eBR2a)

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