BrdU系列产品精选！

关于BrdU              
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU)，中文名为5-溴脱氧尿嘧啶核苷，是一种合成的核苷，胸腺嘧啶(thymidine)的类似物。BrdU普遍使用于检测活组织中的增殖细胞，对研究细胞动力学有重要意义。
BrdU可被正在复制的细胞(S期)所摄取，整合至新合成的DNA链中，可取代胸腺嘧啶(thymidine)。然后可用BrdU特异性的抗体来检测被摄入的BrdU，以此来反应细胞的增殖。DNA须经过变性(denaturation)才可与抗体结合，通常会用酸或热来处理细胞。
 
工欲善其事，必先利其器，BrdU检测工具，您了解多少？
 
BrdU细胞增殖ELISA检测试剂盒
Exalpha公司研发的Frontier™ BrdU 细胞增殖检测分析试剂盒，是一种完全适用于高通量应用、非同位素标记法的ELISA检测试剂盒(可提供显色法和化学发光法)，可检测掺入到贴壁细胞及非贴壁细胞新合成DNA链中的BrdU。
 
试剂盒检测原理
主要以细胞层作为固相载体, 将BrdU摄入微孔板中培养的细胞。在细胞培养的最后2-14小时，把BrdU加入微孔板中，BrdU将被掺入到分裂细胞的DNA中。为确保抗体与BrdU有效结合，需要将细胞固定并做破膜处理，对DNA进行变性。所有操作仅需通过一步固定剂(Fixing solution)处理即可。将BrdUd单克隆抗体加入到板孔中，孵育1小时，未结合的抗体通过洗涤步骤去除，然后加入HRP标记的羊抗小鼠二抗，最后加入HRP底物显色。
 

 

名称	货号	规格	品牌
Frontier™ Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit(比色法)	X1327K1	200 tests	Exalpha
Frontier™ Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit(比色法)	X1327K2	1000 tests	Exalpha
Frontier™ Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit(比色法)	X1327K3	5000 tests	Exalpha
Frontier™ Chemiluminescent Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit（化学发光法）	X1623K1	200 tests	Exalpha
Frontier™ Chemiluminescent Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit（化学发光法）	X1623K2	1000 tests	Exalpha
Frontier™ Chemiluminescent Bromodeoxyuridine (BrdU) Cell Proliferation ELISA Kit（化学发光法）	X1623K3	5000 tests	Exalpha

 
BrdU免疫组化检测试剂盒
BrdU被掺入到分裂细胞的DNA中，然后加生物素化的BrdU特异性抗体孵育，再通过HRP-链亲和素结合物催化DAB底物显色。该试剂盒可用对任何种属的组织进行染色。
 

 

名称	货号	规格	品牌
Frontier™ Bromodeoxyuridine (BrdU) Immunohistochemistry Kit	X1545K	50 slides	Exalpha

 
BrdU流式染色试剂盒
 

 

名称	应用	货号	品牌
BrdU Staining Buffer Set	FC	00-5525	eBioscience
BrdU Staining Kit for Flow Cytometry APC	FC	8817-6600	eBioscience
BrdU Staining Kit for Flow Cytometry eFluor® 450	FC	8848-6600	eBioscience
BrdU Staining Kit for Flow Cytometry FITC	FC	8811-6600	eBioscience
BrdU Staining Kit for Flow Cytometry PE	FC	8812-6600	eBioscience
BrdU Staining Kit for Flow Cytometry PerCP-eFluor® 710	FC	8846-6600	eBioscience

 
BrdU抗体
 

 

名称	货号	规格	品牌
Sheep anti BrdU Polyclonal Antibody	A205P	250 µg	Exalpha
Mouse Anti-Bromodeoxyuridine Monoclonal Antibody	X1028	100 µg	Exalpha
Mouse Anti-Bromodeoxyuridine Monoclonal Antibody(FITC conjugate)	X1543M	100 µg	Exalpha
RAT ANTI BrdU	MCA2060	0.2 mg	AbD Serotec
RAT ANTI BrdU:Alexa Fluor® 488	MCA2060A488	100tests	AbD Serotec
RAT ANTI BrdU:Biotin	MCA2060B	0.1 mg	AbD Serotec
RAT ANTI BrdU:FITC	MCA2060F	0.1 mg	AbD Serotec
RAT ANTI BrdU:FITC	MCA2060FT	0.5 ml	AbD Serotec
RAT ANTI BrdU	MCA2060GA	0.1 mg	AbD Serotec
RAT ANTI BrdU:HRP	MCA2060P	0.5 mg	AbD Serotec
RAT ANTI BrdU:HRP	MCA2060PA	0.1 mg	AbD Serotec
RAT ANTI BrdU	MCA2060T	20 µg	AbD Serotec
MOUSE ANTI BrdU	MCA2483	0.2 mg	AbD Serotec
MOUSE ANTI BrdU:FITC	MCA2483FA	50 µg	AbD Serotec
MOUSE ANTI BrdU	MCA2483T	20 µg	AbD Serotec
RAT ANTI BrdU	OBT0030	0.5 mg	AbD Serotec
RAT ANTI BrdU	OBT0030CX	0.25 ml	AbD Serotec
RAT ANTI BrdU:FITC	OBT0030F	1 ml	AbD Serotec
RAT ANTI BrdU	OBT0030G	0.25 mg	AbD Serotec
RAT ANTI BrdU	OBT0030S	5 ml	AbD Serotec
BrdU [MoBu-1] antibody (Biotin)	GTX10362	100μg	GeneTex
BrdU antibody [BU6-4]	GTX12219	50μg	GeneTex
BrdU antibody	GTX128091	100μl	GeneTex
BrdU antibody [BU1/75 (ICR1)]	GTX26326	100μg	GeneTex
BrdU antibody [BU1/75 (ICR1)] (FITC)	GTX27384	250μl	GeneTex
BrdU antibody [B33.1]	GTX27781	500μl	GeneTex
BrdU antibody [B684M]	GTX44314	100μg	GeneTex
BrdU antibody [IIB5] (FITC)	GTX50089	500μl	GeneTex
BrdU, mAb (2B1)	ADI-MSA-200-E	1*100ug	ENZO
5-Bromodeoxyuridine, mAb (PRB-1) (biotin conjugate)	ALX-804-197B-T060	60 tests	ENZO
5-Bromodeoxyuridine, mAb (PRB-1) (fluorescein conjugate)	ALX-804-197L-T060	60 tests	ENZO
Anti-BrdU APC	17-5071	25/100tests	eBioscience
Anti-BrdU eFluor® 450	48-5071	25/100tests	eBioscience
Anti-BrdU FITC	11-5071	25/100tests	eBioscience
Anti-BrdU PE	12-5071	25/100tests	eBioscience
Anti-BrdU PerCP-eFluor® 710	46-5071	25/100tests	eBioscience
Anti-BrdU Purified	14-5071	25/100ug	eBioscience