ProSci热销产品推荐 — CXCR4 Antibody【1009】

CXCR4是一种含七个跨膜结构域的G蛋白偶联受体（GPCR），是基质衍生因子-1（SDF-1/CXCL12）的特异性CXC趋化因子受体｡CXCR4最初被发现的身份是HIV入侵CD4+ T细胞的共受体之一，阻断CXCR4有望成为HIV治疗的新策略｡CXCR4信号通路在造血干细胞的迁移､增殖和静息及其在骨髓中的滞留中发挥重要作用，并介导B细胞CD20的上调｡CXCR4在23种以上的肿瘤中高表达（包括乳腺癌､卵巢癌､黑色素瘤和前列腺癌），而在正常组织中表达极低或不表达,其表达与肿瘤的存活､生长和向高SDF-1/CXCL12表达组织（如肺､肝､骨髓）的转移密切相关｡

ProSci的CXCR4多克隆抗体（货号1009）是肿瘤微环境与免疫研究领域的理想工具｡该抗体以人CXCR4 isoform b氨基端14个氨基酸肽段为免疫原，经肽段亲和柱纯化，可识别人、小鼠、大鼠来源的CXCR4蛋白，并预测对牛(86%)和猪(71%)具有交叉反应性｡经验证可用于Western Blot、免疫组化(IHC-P)、免疫荧光(IF)、免疫细胞化学(ICC)、流式细胞术(FC)和ELISA等多种应用，覆盖从蛋白表达检测到细胞定位分析的完整实验需求｡此外，该抗体通过了独立抗体验证、siRNA敲低验证及重组蛋白检测等多重特异性验证，数据可靠，品质值得信赖｡

产品信息

实验图例

图1. Western Blot Validation of CXCR4 in HeLa Cells

Loading: 15 ug of lysates per lane. Antibodies: 1009 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

图2. Immunofluorescence Validation of CXCR4 in HeLa Cells

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with 1009 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.

图3. Immunohistochemistry Validation of CXCR4 in Human Spleen

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (1009) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

ProSci致力于为生命科学研究者提供高品质､经过严格验证的抗体产品，以独立抗体验证和多维度特异性检测为特色，为您的科研提供坚实可靠的数据支撑｡

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