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BioXcell热销品BE0101：小鼠PD-L1单克隆抗体（Clone: 10F.9G2™）
人阅读 发布时间：2023-07-05 15:07
产品描述：
The 10F.9G2™ monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1. The 10F.9G2™ antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).
BioXcell InVivoMab anti-mouse PD-L1 (B7-H1) 10F.9G2单克隆抗体与小鼠PD-L1（也称为B7-H1或CD274）反应。PD-L1是属于Ig超家族的B7家族的I型跨膜蛋白，分子量为40kDa。PD-L1在T淋巴细胞、B淋巴细胞、NK细胞、树突状细胞以及IFNγ刺激的单核细胞、上皮细胞和内皮细胞上表达。PD-L1与CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上的受体PD-1结合。PD-L1与PD-1的结合导致抑制TCR介导的T细胞增殖和细胞因子产生。PD-L1被认为在肿瘤免疫逃避中起着重要作用。诱导的PD-L1表达在许多肿瘤中很常见，并导致肿瘤细胞对CD8 T细胞介导的裂解的抗性增加。在黑色素瘤的小鼠模型中，可以通过用阻断PD-L1和PD-1之间相互作用的抗体处理来暂时肿瘤生长。BioXcell InVivoMab anti-mouse PD-L1 (B7-H1) 10F.9G2抗体已被证明可以阻断PD-L1和PD-1之间以及PD-L1和B7-1之间的相互作用（CD80）。
产品详情：
| 产品名称 | InVivoMAb anti-mouse PD-L1 (B7-H1) / 小鼠PD-L1单克隆抗体 |
| 产品货号 |
BE0101 |
| 产品规格 |
1/5/25/50/100mg |
| 反应种属 |
Mouse |
| 克隆号 |
10F.9G2 |
| 同种型 |
Rat IgG2a, κ |
| 免疫原 |
Mouse CD274 |
| 实验应用 |
in vivo PD-L1 blockade Immunofluorescence Immunohistochemistry (frozen) Flow cytometry Western blot |
| 产品形式 |
PBS, pH 6.5,Contains no stabilizers or preservatives |
| 纯度 |
>95%, Determined by SDS-PAGE |
| 无菌处理 |
0.2 µm filtration |
| 纯化方式 |
Protein G |
| RRID |
AB_10949073 |
| 分子量 |
150 kDa |
| 保存条件 |
抗体原液保存在4°C，不能冷冻保存。 |
| 推荐同型对照 |
InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin（货号BE0090） |
| 推荐抗体稀释液 |
InVivoPure pH 6.5 Dilution Buffer（货号IP0065） |
该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：
| 应用 |
文章 |
| 体内PD-L1信号阻断 (in vivo PD-L1 blockade) |
1. Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100. 2. Stathopoulou, C., et al. (2018). 'PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells' Immunity 49(2): 247-263 e247. 3. Jaworska, K., et al. (2015). 'Both PD-1 ligands protect the kidney from ischemia reperfusion injury' J Immunol 194(1): 325-333. 4. Kim, J., et al. (2015). 'Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help' Nat Commun 6: 7994. 5. Zander, R. A., et al. (2015). 'PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity' Cell Host Microbe 17(5): 628-641. 6. Tkachev, V., et al. (2015). 'Programmed death-1 controls T cell survival by regulating oxidative metabolism' J Immunol 194(12): 5789-5800. 7. Twyman-Saint Victor, C., et al. (2015). 'Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer' Nature 520(7547): 373-377.
|
| 体内PD-L1阻断，流式细胞术 (in vivo PD-L1 blockade, Flow Cytometry) |
1. loulou, M., et al. (2016). 'Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells' Nat Commun 7: 10579. 2. Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811. 3. Rutigliano, J. A., et al. (2014). 'Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells' J Virol 88(3): 1636-1651. |
| 体内PD-L1阻断，免疫荧光 (in vivo PD-L1 blockade, Immunofluorescence) |
1.Willimsky, G., et al. (2013). 'Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance' J Clin Invest 123(3): 1032-1043. |
| 免疫组织化学（冷冻），免疫荧光 (Immunohistochemistry (frozen), Immunofluorescence ) |
1.Riella, L. V., et al. (2011). 'Essential role of PDL1 expression on nonhematopoietic donor cells in acquired tolerance to vascularized cardiac allografts' Am J Transplant 11(4): 832-840. |
如需购买Bioxcell BE0101小鼠PD-L1单克隆抗体或Bioxcell其它产品，请咨询 BioXcell 中国授权代理——欣博盛生物
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