BioXCell：InVivoMAb anti-mouse CD47 (IAP)

产品货号

BE0270

产品规格

1/5/25/50/100mg

抗体亚型

Rat IgG2a, κ

推荐同型对照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（货号：BE0089）

推荐抗体稀释液

InVivoPure™ pH 7.0 Dilution Buffer（货号：IP0070）

免疫原

Intact CD47 purified from placenta

应用

in vivo CD47 blockade

in vitro CD47 blockade

Immunofluorescence

产品形式

PBS , pH 7.0  

Contains no stabilizers or preservatives

内毒素水平

<2EU/mg (<0.002EU/μg)

使用 LAL gel clotting 测定

纯度

>95% Determined by SDS-PAGE

无菌处理

0.2 μM filtered

生产形式

从组织培养上清液中纯化得到。

纯化形式

Protein G

RRID

AB_2687793

分子量大小

150 kDa

保存条件

抗体原溶液应保存在4°C条件下，不要冷冻保存。

in vitro CD47 blockade

Liu, M., et al. (2019). “Metabolic rewiring of macrophages by CpG potentiates clearance of cancer cells and overcomes tumor-expressed CD47-mediated ‘don’t-eat-me’ signal.” Nat Immunol 20(3): 265-275.

in vivo CD47 blockade

Pan, Y., et al. (2019). “Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer.” J Hematol Oncol 12(1): 124.

Immunofluorescence

Hsieh, C. P., et al. (2015). “Deficits in cerebellar granule cell development and social interactions in CD47 knockout mice.” Dev Neurobiol 75(5): 463-484.