FNA_200811_Neo_ERseeing

 

ERseeing is irreversible ERlabelingdye and suitable for long-term liveimaging.

Comparedto conventional ERdyes such as Glibenclamide-based dye,ERseeing exhibits very low pharmacological effect and high retentivity.Therefore it can visualize ER structure even after medium change or drug treatment.

 

 

 

	Conventional dye	ERseeing
Pharmacological effect	High	Low
Observation after wash out	Lowsignals	Possible
Fixation after staining	Partial staining /Low signals	Possible

 

ER specificity

 

HeLa cells were stained with ERseeing(100 nM) and organelle markers, Glibencramidetype ER staining, lysosomal staining, mitochondrial staining. ERseeing was highly overlapped with conventional Glibencramidetype ER staining but not correlated with lysosome marker or mitochondria marker.

 

Comparison between ERseeingand conventional dye

 

HeLa cells were treated with ERseeingor Glibenclamide-based dye for 15 min and observed without washout (Left). Both reagents show ER staining. After that, cells were washed by PBS, added fresh media containing 10% FBS and observed again. While the Glibenclamide-based dye showed a very weak signal from the cells, ERseeingmaintains a good signal from ER. ERseeingis suitable for long-term imaging after medium changes.

 

Product information

Exmax/Emmax= 509 / 524 nm.

Commercial FITC filter sets are compatible.                                                        [ Manufacturer : FNA ]

 

Product Name	Code	Size	Storage	Price
ERseeing	FDV-0038	10nmol	-20°C

 

 

NOTE	※All products here are research use only, not for diagnostic use.	※Company name and product name are trademark or registered mark.
	※Specs might be changed for improvement without notice.	※Please contact your local distributors for orders, quote request and inquiry.

 

 

 

 

 

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