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870 人阅读发布时间:2019-11-07 13:53
氧化应激可通过检测活性氧(ROS)来直接检测,也可通过检测ROS过量产生时脂质、蛋白、核酸等相关的损伤来间接检测。虽然直接检测ROS是一种理想的方法,但ROS不稳定,所以研究者通常偏向检测一些相对稳定的损伤标志物。为了帮助您来筛选决定哪一种损伤标志物在您的研究中最合适,Cayman提供多种常见氧化损伤生物标志物检测试剂盒供您选择。并且Cayman与欣博盛生物联合推出氧化应激检测试剂盒限时6折优惠活动,做氧化应激相关研究的您不要错过!
活性氧(ROS)检测
| 货号 | 产品名称 | 规格 | 检测 | 有用信息 |
| 600050 | Hydrogen Peroxide Cell-Based Assay Kit |
96 wells | Extracellular H2O2 |
Utilizes ADHP, a sensitive and stable probe for H2O2, and includes catalase to check assay specificity |
| 701600 | Mitochondrial ROS Detection Assay Kit |
96 wells | Mitochondrial ROS |
Utilizes a mitochondria-specific, fluorescent ROS detection reagent to measure the production of ROS under specific conditions; includes Antimycin A as a positive control |
| 10010895 | Xanthine Oxidase Fluorometric Assay Kit |
96 wells | XO activity | Based on a multistep enzymatic reaction in which the H2O2 produced when XO oxidizes hypoxanthine reacts with ADHP |
| 601290 | ROS Detection Cell-Based Assay Kit (DHE) |
96 wells | ROS | Utilizes the redox-sensitive probe DHE as a substrate for O2•and H2O2; includes positive and negative controls for ROS generation and scavenging, respectively |
活性氮(RNS)检测
| 货号 | 产品名称 | 规格 | 检测 | 有用信息 |
| 780051 | Nitrate/Nitrite Fluorometric Assay Kit |
2 x 96 wells | NO• metabolites | Utilizes DAN instead of Griess reagents, which enables 20-fold increased sensitivity over the colorimetric version; allows for detection of low concentrations of NO2- and NO3- (minimum detectable quantity of NO2/NO3 is ~50 nM) |
| 760871 | Nitrate/Nitrite Colorimetric Assay Kit (LDH method) |
96 wells | In vitro NOS activity and NO• metabolites |
Use to analyze NO2- and NO3- from an in vitro NOS assay in which excess NADPH has been added; an extra step is included in the protocol that uses LDH to remove the excess NADPH |
| 780001 | Nitrate/Nitrite Colorimetric Assay Kit |
2 x 96 wells | NO• metabolites | Uses a small amount of added NADPH in conjunction with a catalytic system for recycling spent NADP+ back to NADPH to avoid NADPH interference with the chemistry of the Griess reagents; works well for the analysis of fluids such as plasma and urine, but cannot be used to analyze NO2- and NO3- from an in vitro assay of OS in which excess NADPH has been added |
| 781001 | NOS Activity Assay Kit |
1 ea | NOS activity | Monitors the conversion of radiolabeled arginine to citrulline by NOS |
DNA/RNA损伤检测
| 货号 | 产品名称 | 规格 | 检测 | 有用信息 |
| 501130 | DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit |
96 wells | 8-hydroxy-2’ -Deoxyguanosine and 8-hydroxyguanosine |
Monoclonal antibody (clone 7E6.9) enables detection of 8-hydroxy-2’ -deoxyguanosine (DNA oxidative damage marker) and 8-hydroxyguanosine (RNA damage marker) with equal selectivity and sensitivity; correlates with LC/MS measurements of a combination of 8- hydroxy-2'-deoxyguanosine and 8- hydroxyguanosine |
| 589320 | DNA/RNA Oxidative Damage (High Sensitivity) ELISA Kit |
96 wells | 8-hydroxy-2’ -Deoxyguanosine, 8-hydroxyguanosine,and 8-hydroxyguanine |
Monoclonal antibody enables detection of 8-hydroxy-2’-deoxyguanosine (DNA oxidative damage marker), 8-hydroxyguanosine (RNA damage marker), and 8-hydroxyguanine (DNA/RNA damage marker) with selectivity nd sensitivity highest for 8-hydroxy-2’ -deoxyguanosine; correlates with LC/MS measurements of 8-hydroxy-2'-deoxyguanosine, though high slope indicates other unknown species are detected |
蛋白氧化及硝化检测
| 货号 | 产品名称 | 规格 | 检测 | 有用信息 |
| 10005020 | Protein Carbonyl Colorimetric Assay Kit |
96 wells | Protein carbonyl content |
Utilizes the reaction between DNPH and protein carbonyls as a readout of protein oxidation |
| 10006518 | S-Nitrosylated Protein Detection Kit (Biotin Switch) |
1 ea | S-NO proteins | Utilizes a modified 'Biotin-switch' method to directly tag S-NO proteins |
| 601220 | Nitrotyrosine IP Kit | 1 ea | Nitrated tyrosine content |
Utilizes a sorbent coupled with a nitrotyrosine monoclonal antibody to capture and pulldown nitrated proteins |
| 10010721 | Sglutathionylated Protein Detection Kit |
1 ea | Protein-PSSG adducts |
Utilizes a modified 'Biotin-switch' method to directly tag protein-PSSG adducts |
| 600160 | Methionine Sulfoxide Immunoblotting Kit | 1 ea | Proteins containing MetO residues |
Utilizes a MetO polyclonal antibody isolated from rabbit serum that is specific for MetO and demonstrates minimal cross reactivity with methionine sulfone |
脂质过氧化检测
| 货号 | 产品名称 | 规格 | 检测 | 有用信息 |
| 10009055 | TBARS Assay Kit | 96 wells | MDA-TBA adduct |
Standard method to determine lipid peroxidation; reaction yields higher sensitivity when measured fluorometrically, but a colorimetric method is included as an option |
| 516351 | 8-Isoprostane ELISA Kit |
96 wells | 8-Isoprostane | Overnight assay (incubation time = 18 hours) uses AChE tracer; assay range 0.8-500 pg/ml; detection limit (80% B/B0) of ~3 pg/ml |
| 500431 | STAT-8-Isoprostane ELISA Kit |
96 wells | 8-Isoprostane | Extremely rapid assay (results in ~2 hours) uses an AP tracer; assay range 23.4-3,000 pg/ml; detection limit (80% B/B0) of ~45 pg/ml |
| 516360 | 8-Isoprostane Express ELISA Kit |
96 wells | 8-Isoprostane | 4 hour assay uses AChE tracer; assay range 2.5-1,500pg/ml; detection limit (80% B/B0) of ~10 pg/ml |
| 700870 | TBARS (TCA Method) Assay Kit |
96 wells | MDA-TBA adduct |
Offers the advantage of improved sample processing and reduced working volumes by incorporating a TCA precipitation procedure; maintains same reliability and accuracy as the original TBARS Assay; includes sample acid precipitation protocol to avoid confounding soluble TBARS |
| 705002 | Lipid Hydroperoxide (LPO) Assay Kit |
100 dtn | LPOs | Designed for use with a single-tube spectrophotometer to read the results |
| 705003 | Lipid Hydroperoxide (LPO) Assay Kit (96 well) |
96 wells | LPOs | Designed for use with a reusable glass plate |
其他相关试剂盒
| 货号 | 产品名称 | 规格 |
| 703302 | Glutathione S-Transferase Assay Kit | 96 wells |
| 700340 | Thiol Detection Assay Kit | 96 wells |
| 706002 | Superoxide Dismutase Assay Kit | 96 wells |
| 10007892 | Thioredoxin Reductase Colorimetric Assay Kit | 96 wells |
| 389549 | Nitrotyrosine Affinity Sorbent | 200 µg |
| 501110 | 8-Isoprostane Affinity Purification Kit | 1 ea |
| 600320 | Sulfenylated Protein Cell-Based Detection Kit | 96 wells |
| 600360 | Glutathione Cell-Based Detection Kit (Blue Fluorescence) | 96 wells |
| 601460 | Hydrogen Peroxide Ratiometric MaxSpec® Kit | 1 ea |
| 700910 | Catalase Assay Kit (without Hydrogen Peroxide) | 96 wells |
| 703002 | Glutathione Assay Kit | 96 wells |
| 703102 | Glutathione Peroxidase Assay Kit | 96 wells |
| 703202 | Glutathione Reductase Assay Kit | 96 wells |
| 707002 | Catalase Assay Kit | 96 wells |
| 709001 | Antioxidant Assay Kit | 96 wells |
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