特异性Fe2+荧光探针 FeRhoNoxTM-1

4487 人阅读发布时间：2018-05-16 16:10

Specific Fluorescent probe for Fe2+FeRhoNox™-1
特异性Fe2+荧光探针 FeRhoNoxTM-1
FeRhoNoxTM-1是特异性检测Fe2+的荧光探针
该探针能够特异性地与高尔基体的Fe2+反应。能检测活细胞高尔基体的Fe2+

Features	产品特征
●Specific detection of Fe2+	● 特异性检测Fe2+
●Applicable to live cell imaging of labile Fe2+	● 适用于不稳定的Fe2+的活细胞成像
●For detection of Fe2+ in aqueous media	● 适合于检测水溶性基质里的Fe2+
●For detection of labile iron in living cells	● 适合于检测活细胞内不稳定的离子


enceappears aroResponse of FeRhoNox™-1 to Fe2+. Reacting FeRhoNox™-1 with Fe2+for 1 h at 37℃, peak of the fluorescund 575 nm.	Fe2+detection by FeRhoNox™-1in HepG2 cells. HepG2 cells preincubated with/without Fe(NH4)2(SO4)2(FAS: 100 µM) for 0.5 hrwas incubated with 5 µM of FeRhoNox™-1 for 1 hr.

The selectivity of FeRhoNox™-1.FeRhoNox™-1 can react with only Fe2+selectively.	Comparison between FeRhoNox™-1’s and Golgi Marker’s staining images. The Merge image shows that FeRhoNox™-1 selectively reacts with Fe2+in Golgi apparatus.

Code no.	Product Name	Target	Size
GC 901	FeRhoNox™-1	Fe2+in aqueous media, labile Fe2+in living cells	50μg×10

Application examples using FeRhoNox™-1

To HepG2 cell


① - Fe2+ : not added Fe2+ （100 μM Ferrous ammoniumsulfate）	Bpy: cell permeable Fe2+ selective chelator (2,20-bipyridyl).
② +Fe2+ : added Fe2+
③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator
② shows increasing of FeRhoNox™-1’s fluorescence intensity and
③showsdecreasing of it. These results indicate that FeRhoNox™-1 reacts with Fe2+ selectively.

To HEK293 cell

①-Fe2+: not added Fe2+ （100 μMFerrous ammonium sulfate）

②+Fe2+ : added Fe2+

③+Fe2+ +Bpy: added Fe2+and Bpy(1 mM) as Fe2+ chelator

②shows increasing of FeRhoNox™-1’s fluorescence intensity and

③shows decreasing of it. These results indicate that FeRhoNox™-1
reacts with Fe2+ selectively.

Detection of internal Fe2+

Since the fluorescence intensity on
②appears decreased compared to
①by adding “Bpy”, FeRhoNox™-1
detects internal iron(II).

详情请联系 GORYO CHEMICAL 全国授权代理-欣博盛生物科技
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